革兰氏染液,Gram Stain Kit

革兰氏染液,Gram Stain Kit

【Numbering】20211227-172 【CAS】CAS
【Item No.】20211227-172-5 【specification】
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【产品介绍】本试剂盒用于细菌等微生物的形态染色,作为细菌等微生物鉴定的依据,染色后镜检结果为革兰氏阴性菌呈红色,革兰氏阳性菌呈紫色。具有操作简便、方法可靠,试剂性质稳定、保质期长,结果易于观察和判断等优点。

【染色原理】革兰染色法的意义就在于鉴别细菌,把众多的细菌分为两大类,革兰氏阳性菌和革兰氏阴性菌。首先,细菌经初染剂结晶紫初染成蓝紫色,再加媒染剂与结晶紫结合成结晶紫-碘的复合物,从而增强了染料与细菌的结合力。革兰氏阳性细菌(G+)的细胞壁主要由肽聚糖形成的网状结构组成,壁厚、类脂含量低,用脱色剂脱色时细胞壁脱水、使肽聚糖层的网状结构孔径缩小,透性降低,从而使结晶紫-碘的复合物不易被洗脱而保留在细胞内,经脱色和复染后仍保留初染剂的蓝紫色。而革兰氏阴性菌(G-)细胞壁类脂含量多,肽聚糖层较薄且肽聚糖为平面片层结构,易被脱色剂溶解,使细胞壁通透性增高,结合的染料复合物容易泄漏,细菌被脱色为无色,再经复染液复染成红色。

【所需仪器】光学显微镜、玻片、染缸、滴管、秒表、记号笔等。

参数信息
外观状态: 固体或粉末
质量指标: 95%+
溶解条件: 有机溶剂/水
CAS号: N/A
分子量: N/A
储存条件: -20℃避光保存
储存时间: 1年
运输条件: 室温2周
生产厂家: 苏州北科纳米科技有限公司

Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance

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