Epoxy-Sepharose FF
| 【Numbering】BK-NRPB12L | 【CAS】CAS | ||
| 【Item No.】BK-NRPB12L-500 | 【specification】 | ||
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Product name: Epoxy-Sepharose FF
English name: Epoxy Activated NUPharose Fast Flow, Epoxy NUPharose FF
Catalog Number: NRPB12L
Specifications: 1ml, 10 ml, 100 ml, 1L, special specifications customized
Transportation: 2 ~ 25℃, normal pressure, avoid light
Storage: 100% acetone, -20℃
Shelf life: 1 year
Related introduction:
Epoxy-Sepharose FF is an activation chromatography separation medium intermediate formed by bonding epoxy groups on agarose microspheres. The product retains the excellent hydrophilicity of agarose and the large network structure, has good compatibility with biologically active macromolecules, and has high activated group density. It is suitable for carrying amino, hydroxyl, sulfhydryl and other groups. The coupling of the ligands of the group has high coupling efficiency.
Technical index:
Exterior
Milky white translucent gelatinous gel
Matrix
4% agarose gel
Ligand
Epoxy
Ligand Density
≥15 μmol/mL
Protein coupling amount
≥10 mg/ml IgG
Maximum flow rate
300 cm/h
Back pressure resistant
0.3 MPa
Recommended flow rate
50 ~ 100 cm/h
Particle size range
60 ~ 180μm
Operating temperature
4 ~ 40℃
Matrix pH stability
3 ~ 12 (long time); 2 ~ 14 (short time)
Matrix chemical stability
Stable in the following solutions: commonly used aqueous buffer; 1 mol/L NaOH; 1 mol/L acetic acid (pH 4.0); 6 mol/L guanidine hydrochloride; 70% ethanol.
Instructions:
1. Before ligand coupling, prepare all samples, solutions, buffers, etc., to minimize the gap between each step.
2. Wash more than 10 volumes with distilled water before coupling, and remove the preservation solution.
3. Choose the coupling solution system according to the target ligand. Generally, the pH for sulfhydryl coupling is 7-9, the pH for amino coupling is 9-11, and the pH for hydroxyl coupling is >11.
4. It can be coupled at 20-40°C. Generally, increasing the temperature can accelerate the coupling. In the case of poor coupling effect, the temperature can be increased to 35°C or more, but the stability of the ligand must be considered.
5. The coupling time depends on the reaction speed, usually it can be coupled overnight (16 h).
6. After coupling the ligand, the remaining epoxy groups need to be blocked. Use cysteine, ethanolamine, glycine and other small molecule reagents to block for several hours, such as 0.2 M Na2CO3, 0.1 M ethanolamine, pH 10, 37℃ for 16 h .
7. After coupling, wash with pH 8-9, 0.1 mol/LTris-HCl buffer and pH 4-5, 0.1 M acetic acid-sodium acetate buffer in turn, and cycle three times to remove uncoupled residual ligands.
8. The coupled gel can be stored in a solution containing a bacteriostatic agent, such as 20% ethanol.
Message
English name: Epoxy Activated NUPharose Fast Flow, Epoxy NUPharose FF
Catalog Number: NRPB12L
Specifications: 1ml, 10 ml, 100 ml, 1L, special specifications customized
Transportation: 2 ~ 25℃, normal pressure, avoid light
Storage: 100% acetone, -20℃
Shelf life: 1 year
Related introduction:
Epoxy-Sepharose FF is an activation chromatography separation medium intermediate formed by bonding epoxy groups on agarose microspheres. The product retains the excellent hydrophilicity of agarose and the large network structure, has good compatibility with biologically active macromolecules, and has high activated group density. It is suitable for carrying amino, hydroxyl, sulfhydryl and other groups. The coupling of the ligands of the group has high coupling efficiency.
Technical index:
Exterior
Milky white translucent gelatinous gel
Matrix
4% agarose gel
Ligand
Epoxy
Ligand Density
≥15 μmol/mL
Protein coupling amount
≥10 mg/ml IgG
Maximum flow rate
300 cm/h
Back pressure resistant
0.3 MPa
Recommended flow rate
50 ~ 100 cm/h
Particle size range
60 ~ 180μm
Operating temperature
4 ~ 40℃
Matrix pH stability
3 ~ 12 (long time); 2 ~ 14 (short time)
Matrix chemical stability
Stable in the following solutions: commonly used aqueous buffer; 1 mol/L NaOH; 1 mol/L acetic acid (pH 4.0); 6 mol/L guanidine hydrochloride; 70% ethanol.
Instructions:
1. Before ligand coupling, prepare all samples, solutions, buffers, etc., to minimize the gap between each step.
2. Wash more than 10 volumes with distilled water before coupling, and remove the preservation solution.
3. Choose the coupling solution system according to the target ligand. Generally, the pH for sulfhydryl coupling is 7-9, the pH for amino coupling is 9-11, and the pH for hydroxyl coupling is >11.
4. It can be coupled at 20-40°C. Generally, increasing the temperature can accelerate the coupling. In the case of poor coupling effect, the temperature can be increased to 35°C or more, but the stability of the ligand must be considered.
5. The coupling time depends on the reaction speed, usually it can be coupled overnight (16 h).
6. After coupling the ligand, the remaining epoxy groups need to be blocked. Use cysteine, ethanolamine, glycine and other small molecule reagents to block for several hours, such as 0.2 M Na2CO3, 0.1 M ethanolamine, pH 10, 37℃ for 16 h .
7. After coupling, wash with pH 8-9, 0.1 mol/LTris-HCl buffer and pH 4-5, 0.1 M acetic acid-sodium acetate buffer in turn, and cycle three times to remove uncoupled residual ligands.
8. The coupled gel can be stored in a solution containing a bacteriostatic agent, such as 20% ethanol.
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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