Recombinant Human acid fibroblast growth Factor (RH-AFGF)










Specification: 10 μg, 100 μg, custom-made






Form: Lyophilized powder, 10 mM PBS (1 mM Na2HPO4, 9 mM NaH2PO4, 150 mM NaCl, pH 6.0)






Sub-quantity: 16.0kda






Isoelectric point: 7.73






Purity: ≥95% (SDS-PAGE test)






A280nm (1 mg/mL) : 1.09






Endotoxin: ≤5 EU/mg






Source: E. coli






Storage condition: -20℃ storage






Effective period: 3 years






Activity: EC50≤0.5 ng/ mL, corresponding to protein activity ≥2.0×106 U/mg (dose dependent proliferation of mouse BALB/ C 3T3 cells)






















Related introduction:






The acidic Growth Factor (aFGF), also known as FGF1, belongs to the FGF family, is involved in the repair of tissue damage, and has the role of nutrition and promotion of division and differentiation for a variety of cells derived from mesoderm and neuroectoderm. Such as BALB/ C 3T3 fibroblasts, capillary and endocardial cells, myoblasts, vascular smooth muscle cells, mesodermal cells, neuroglia and astrocytes, adrenal cortical cells, etc., have a wide range of biological effects and potential clinical application value.














AFGF molecule contains heparin-binding region, which can increase the activity of SOME cells after the combination of aFGF and heparin, and a certain amount of heparin can increase the stability of aFGF and prevent the degeneration of aFGF by heat, extreme pH and protease hydrolysis.






Recombinant human acidic fibroblast growth factor (RH-AFGF) expressed by EScherichia coli is a non-glycosylated single-chain protein containing 141 amino acids with a molecular weight of 16.0 kDa.














Picture 20. The JPG






M: Protein molecular weight standard






Lane 1: rh - aFGF














Notes:






1) After dissolving, place it on an ice bath and use it as soon as possible;






2) If it cannot be used up at one time, please prepare mother liquor before use and divide it into small specifications (ensure the amount of one test) and store it at -65℃ to avoid repeated freeze-thaw effects on protein activity






3) Avoid the use of strong acid, strong alkali, strong oxidant, high concentration of organic solvent and other easy to cause protein denaturation reagents;






4) This product is for scientific research only.






Picture 21. The JPG






Rh-afgf stimulated BALB/ C 3T3 cell proliferation

Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance

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