Recombinant Peptic Streptococcus protein L
| 【Numbering】BK-NRPA21S | 【CAS】CAS | ||
| 【Item No.】BK-NRPA21S-100 | 【specification】 | ||
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Name: Recombinant Peptic Streptococcus Protein L
Specifications: 1 mg, 10 mg, 100 mg, 500 mg
Product form: Lyophilized powder, the solution before lyophilization is 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4) solution
Molecular weight: 46 kDa
Isoelectric point: 4.73
Purity: ≥95% (SDS-PAGE detection)
Source: Escherichia coli
Storage conditions: -20℃ storage
Validity period: 3 years
Related introduction:
Protein L is derived from Peptostreptococcus magnus. It is a protein that can bind to a wide range of immunoglobulins. The protein only binds to the κ light chain of the antibody without affecting the antigen binding site of the antibody. One feature makes it more widely combined with antibodies from various sources and subclasses compared with protein A or protein G. It does not bind to immunoglobulins derived from cattle, goats or sheep, and can be used to purify monoclonal antibodies from culture broth supplemented with BSA or FCS. The natural protein L is composed of 719 amino acids, does not contain disulfide bonds, and is an acidic molecule PI 4. It binds to the light chain (L chain) of IgG. Protein L mainly has affinity with Kappa light chain. Protein L has affinity for different IgG, IgM, IgA, IgE and IgD. Protein L binds to the Kappa region of the light chain and has no affinity with lambda. The affinity sites are in the I, III, and IV regions of the light chain.
Human immunoglobulin light chain contains two subtypes, Lambda and Kappa. About 60% of IgG is kappa and 40% of IgG contains Lambda. Before establishing a purification method, it is best to identify the type of light chain and select the correct chromatographic medium for separation and purification.
M: protein molecular weight standard
Lane 1: Recombinant Peptostreptococcal Protein L
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
Message
Specifications: 1 mg, 10 mg, 100 mg, 500 mg
Product form: Lyophilized powder, the solution before lyophilization is 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4) solution
Molecular weight: 46 kDa
Isoelectric point: 4.73
Purity: ≥95% (SDS-PAGE detection)
Source: Escherichia coli
Storage conditions: -20℃ storage
Validity period: 3 years
Related introduction:
Protein L is derived from Peptostreptococcus magnus. It is a protein that can bind to a wide range of immunoglobulins. The protein only binds to the κ light chain of the antibody without affecting the antigen binding site of the antibody. One feature makes it more widely combined with antibodies from various sources and subclasses compared with protein A or protein G. It does not bind to immunoglobulins derived from cattle, goats or sheep, and can be used to purify monoclonal antibodies from culture broth supplemented with BSA or FCS. The natural protein L is composed of 719 amino acids, does not contain disulfide bonds, and is an acidic molecule PI 4. It binds to the light chain (L chain) of IgG. Protein L mainly has affinity with Kappa light chain. Protein L has affinity for different IgG, IgM, IgA, IgE and IgD. Protein L binds to the Kappa region of the light chain and has no affinity with lambda. The affinity sites are in the I, III, and IV regions of the light chain.
Human immunoglobulin light chain contains two subtypes, Lambda and Kappa. About 60% of IgG is kappa and 40% of IgG contains Lambda. Before establishing a purification method, it is best to identify the type of light chain and select the correct chromatographic medium for separation and purification.
M: protein molecular weight standard
Lane 1: Recombinant Peptostreptococcal Protein L
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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