Recombinant Streptococcal Protein G

Recombinant Streptococcal Protein G

【Numbering】BK-NRPA06S 【CAS】CAS
【Item No.】BK-NRPA06S-100 【specification】
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Name: Recombinant Streptococcal Protein G


Specification: 1 mg, 10 mg, 100 mg, 500 mg, special order


Product form: freeze-dried powder; the buffer system before freeze-drying is 5 mM PB (pH 7.4)


Molecular weight: 28 kDa


Isoelectric point: 4.79


A280nm (1 mg/mL): 1.350


Source: Escherichia coli


Purity: ≥95%


Storage conditions: -20℃ storage


Validity period: 3 years


Related introduction:


Streptococcus Protein G (SPG) is a protein in the cell wall of G and C streptococci. It can specifically bind to the Fc segment of immunoglobulin IgG molecules without affecting the binding of its Fab segment to antigen molecules. ability. It can not only bind to all subclasses of human, mouse, and rat IgG, but also binds to guinea pig, rabbit, goat, cow, sheep, and horse IgG, which is significantly better than only binding to human and mouse IgG. Staphylococcus Protein A (Staphylococcus Protein A, referred to as SPA).






SPG is extremely versatile, and can be used to separate and purify or remove IgG from serum, purification of monoclonal antibodies, and separation of antigen-antibody complexes. After SPG is combined with agarose gel, affinity fillers for antibody purification can be prepared to separate and purify IgG of different subtypes from serum, ascites, cell culture supernatant, etc.






In addition to the IgG binding domain, natural SPG also has albumin and cell surface binding domains. Recombinant SPG removes albumin and cell surface binding domains to reduce non-specific binding. It has a greater affinity than natural SPG and SPA.


图片10.jpg


M: protein molecular weight standard


Lane 1: r-SPG


Figure 1 SDS-PAGE detection of r-SPG






Precautions:


1) After dissolving, place it on an ice bath and try to use it as soon as possible;


2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity


3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;


4) This product is limited to scientific research use.






Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance

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