Recombinant Alkali Tolerant Protein A
| 【Numbering】BK-NRPA08S | 【CAS】CAS | ||
| 【Item No.】BK-NRPA08S-100 | 【specification】 | ||
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Name: Recombinant Alkali Tolerant Protein A
Specification: 1 mg, 10 mg, 100 mg, 500 mg, special order
Product form: lyophilized powder, the solution before lyophilization is 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4) solution
Molecular weight: 31.2 kDa (SDS-PAGE about 28 kDa)
Isoelectric point: 5.24
A280nm (1 mg/mL): 0.191
Source: Escherichia coli
Purity: ≥95%
Acid resistance:> 0.1 M HCl
Alkali resistance:> 0.1 M NaOH
Heat resistance: 60℃ (1 hour)
Storage conditions: -20℃ storage
Validity period: 3 years
Related introduction:
Recombinant alkali-resistant protein A has modified the protein structure on the basis of natural Staphylococcus aureus protein A. Compared with natural protein A, it removes the binding site with Fab and enhances the specificity of recombinant alkali-resistant protein A and Fc binding. It also improves protein stability and alkali resistance. Recombinant alkali-resistant protein A can be bonded with agarose gel to prepare affinity fillers for antibody purification. It can separate and purify a variety of mammalian subtypes from ascites, serum, cell culture supernatant or cell extracts The antibody or the genetically engineered recombinant protein containing the Fc fragment of the antibody. A cysteine was added to the C-terminus of the recombinant alkali-resistant protein A to enhance its directional covalent bonding with the gel.
Figure 1 HPLC detection of rat-PA
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
M: protein molecular weight standard
Lane 1: Recombinant alkali-resistant protein A
Figure 2 SDS-PAGE detection of rat-PA
Message
Specification: 1 mg, 10 mg, 100 mg, 500 mg, special order
Product form: lyophilized powder, the solution before lyophilization is 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4) solution
Molecular weight: 31.2 kDa (SDS-PAGE about 28 kDa)
Isoelectric point: 5.24
A280nm (1 mg/mL): 0.191
Source: Escherichia coli
Purity: ≥95%
Acid resistance:> 0.1 M HCl
Alkali resistance:> 0.1 M NaOH
Heat resistance: 60℃ (1 hour)
Storage conditions: -20℃ storage
Validity period: 3 years
Related introduction:
Recombinant alkali-resistant protein A has modified the protein structure on the basis of natural Staphylococcus aureus protein A. Compared with natural protein A, it removes the binding site with Fab and enhances the specificity of recombinant alkali-resistant protein A and Fc binding. It also improves protein stability and alkali resistance. Recombinant alkali-resistant protein A can be bonded with agarose gel to prepare affinity fillers for antibody purification. It can separate and purify a variety of mammalian subtypes from ascites, serum, cell culture supernatant or cell extracts The antibody or the genetically engineered recombinant protein containing the Fc fragment of the antibody. A cysteine was added to the C-terminus of the recombinant alkali-resistant protein A to enhance its directional covalent bonding with the gel.
Figure 1 HPLC detection of rat-PA
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
M: protein molecular weight standard
Lane 1: Recombinant alkali-resistant protein A
Figure 2 SDS-PAGE detection of rat-PA
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
Message
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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