Recombinant Staphylococcus aureus protein A
| 【Numbering】BK-NRPA04S | 【CAS】cas | ||
| 【Item No.】BK-NRPA04S-100 | 【specification】 | ||
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Name: Recombinant Staphylococcus aureus protein A
Specification: 1 mg, 10 mg, 100 mg, 500 mg, special order
Product form: freeze-dried powder; the buffer system before freeze-drying is 5 mM PB (pH 7.4)
Molecular weight: 33.6 kDa (SDS-PAGE about 30 kDa)
Isoelectric point: 5.10
A280nm (1 mg/mL): 0.221
Source: Escherichia coli
Purity: ≥95%
Storage conditions: -20℃ storage
Validity period: 3 years
Related introduction:
Protein A is a Staphylococcus aureus cell wall protein that can specifically bind to many mammalian IgG and a small number of IgM and IgA, and mainly binds to the Fc region of immunoglobulin heavy chains. Protein A can be coupled with a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding activity to antibodies. These coupling reactions can be used in ELISA tests, histochemistry, and Western Blot and other antibody detection. After combining protein A with agarose gel, affinity fillers for antibody purification can be prepared, which can separate and purify various mammalian antibodies of different subtypes from ascites, serum, cell culture supernatant or cell extracts Or genetically engineered recombinant protein containing antibody Fc fragment.
M: protein molecular weight standard
Lane 1: Recombinant Protein A
Figure 1 SDS-PAGE detection of r-SPA
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
Message
Specification: 1 mg, 10 mg, 100 mg, 500 mg, special order
Product form: freeze-dried powder; the buffer system before freeze-drying is 5 mM PB (pH 7.4)
Molecular weight: 33.6 kDa (SDS-PAGE about 30 kDa)
Isoelectric point: 5.10
A280nm (1 mg/mL): 0.221
Source: Escherichia coli
Purity: ≥95%
Storage conditions: -20℃ storage
Validity period: 3 years
Related introduction:
Protein A is a Staphylococcus aureus cell wall protein that can specifically bind to many mammalian IgG and a small number of IgM and IgA, and mainly binds to the Fc region of immunoglobulin heavy chains. Protein A can be coupled with a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding activity to antibodies. These coupling reactions can be used in ELISA tests, histochemistry, and Western Blot and other antibody detection. After combining protein A with agarose gel, affinity fillers for antibody purification can be prepared, which can separate and purify various mammalian antibodies of different subtypes from ascites, serum, cell culture supernatant or cell extracts Or genetically engineered recombinant protein containing antibody Fc fragment.
M: protein molecular weight standard
Lane 1: Recombinant Protein A
Figure 1 SDS-PAGE detection of r-SPA
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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