Recombinant Streptavidin

Recombinant Streptavidin

【Numbering】BK-NRPA05S 【CAS】CAS
【Item No.】BK-NRPA05S-100 【specification】
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Name: Recombinant Streptavidin


Specifications: 1 mg, 10 mg, 100 mg, 500 mg


Product form: lyophilized powder, the solution before lyophilization is 5mM PB (4mM Na2HPO4, 1mM NaH2PO4, pH7.4) solution


Molecular weight: 16.7 kDa


Isoelectric point: 7.95


Purity: ≥95% (SDS-PAGE detection)


Concentration determination: 280nm absorption method, molar absorption coefficient is 4.19×104(mol/L)-1·cm-1, that is, c(g/L) = A280nm/2.51


Endotoxin: ≤5EU/mg


Source: Escherichia coli


Storage conditions: -20℃ storage


Validity period: 3 years






Streptavidin (SA) has a high affinity with biotin, and the affinity constant Kd can generally reach 10-15M, and active SA usually exists in the form of tetramers. Because SA does not contain sugar groups, SA has a lower non-specific binding level than avidin in detection applications. It has been widely used in enzyme-linked immunosorbent assays, immunohistochemistry, time-resolved immunofluorescence (TRFIA), Quantitative PCR, single-stranded DNA preparation, biomolecule purification, monoclonal antibody preparation and other biotechnological fields.


However, the super-high affinity of SA and biotin is generally difficult to separate the combined SA from biotin by a gentle method. The conditions that can separate will generally make SA inactive, and the same biotin-labeled antibodies and antigens will also lose activity. Therefore, it is generally believed that the binding of SA and biotin is irreversible. Recombinant streptavidin 2 (r-SA2) is modified at the molecular level on the basis of natural streptavidin to eliminate the binding force to form tetramers. When it is active, it is a monomer, and at the same time greatly weakens the biological The affinity (Kd≈10-7M) of biotin can realize reversible binding and dissociation with biotin, and can be used for gentle separation, purification and recycling of biotinylated proteins, antibodies, nucleic acids and other substances.


图片5.jpg

M: protein molecular weight standard


Lane 1: r-SA2




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